Table 3.
Hepatic functions of different hepatic cell lines and primary mature human hepatocytes (Mat Hep)
| Hepatic function (n = 9) |
mRNA levels (n = 3) Mature hepatic marker |
mRNA levels (n = 3) Immature hepatic marker |
|||||||
| Cell |
Albumin production (ng/h/mg protein) |
Urea production (nmol/h/mg protein) |
Galactose elimination (μmol/h/mg protein) |
Albumin | Transferrin | AAT | HNF4 | GSTπ | AFP |
| Mat Hep | 37.7 ± 7.7 | 91.5 ± 33.7 | 0.58 ± 0.44 | 130 | 245 | 121 | 141 | 535 | ND |
| cBAL08 | 0.3 ± 0.4 | 4.0 ± 1.6 | ND | 0.02 ± 0.01 | 0.20 ± 0.14 | 0.32 ± 0.14 | ND | 1000 ± 565 | 78 ± 61 |
| cBAL111 day 2 |
Undetectable | Undetectable | 0.04 ± 0.02 | 0.02 ± 0.01 | 0.11 ± 0.05 | 0.02 ± 0.01 | 1.3 ± 1.3 | 1944 ± 1010 | 122 ± 11 |
| cBAL111 day 15 |
0.7 ± 0.8 | 8.0 ± 6.6 | 0.11 ± 0.03* | 0.02 ± 0.01 | 0.20 ± 0.06 | 0.05 ± 0.01 | 17.2 ± 5.6* | 1374 ± 671 | 114 ± 10 |
| HepG2 | 2.8 ± 0.3 | 4.7 ± 0.2 | ND | 63 ± 6 | 896 ± 110 | 199 ± 56 | ND | Undetectable | 93353 ± 13228 |
| NKNT-3 reverted | Undetectable | 4.9 ± 9.4 | ND | 0.24 ± 0.22 | 0.16 ± 0.12 | 0.92 ± 1.52 | ND | 952 ± 1164 | 117 ± 166 |
| NKNT-3 unreverted | Undetectable | 2.9 ± 5.2 | ND | 0.42 ± 0.66 | 0.20 ± 0.13 | 0.26 ± 0.34 | ND | 4427 ± 5366 | 65 ± 79 |
MessengerRNA levels are expressed as a percentage of the mean mRNA levels of the two liver samples. ND = not determined
* cBAL111 day 2 versus cBAL111 day 15; P < 0.05