Table 2.

PCR primers and conditions

	Primers
DNA region	Forward	Reverse
COX1	Schisto5'	Schisto3'
	TCTTTRGATCATAAGCG	TAATGCATMGGAAAAAAACA
ITS	ITSF TAACAAGGTTTCCGTAGGTGAA	ITSR TGCTTAAGTTCAGCGGGT

25 μl PCR reactions were carried out using Ready-to-go PCR Beads (Amersham) and 10 pmol of each primer for the DNA region to be amplified as above. Thermal cycling conditions were: 2 min denaturing at 95°C: 40 cycles of 30 s at 95°C, 30 s at 40°C, 2 min at 72°C; followed by final extension period of 7 min at 72°C. Positive amplification was checked by 0.8% Gel Red agarose gel electrophoresis of 3 μl of each amplicon, which were visualised using a UVP gel doc system (Fig 1).