Table 2.
Strains and plasmids used in this study.
| Relevant characteristics | Reference | |
|---|---|---|
| Sinorhizobium meliloti strains | ||
| Rm1021 | SmR derivative of SU47 | Meade et al. (1982) |
| Rm1021Δsku1 | Deletion mutant of Rm1021 where the 0.3 kb SalI-XhoI fragment was removed from sku1 | This study |
| Rm1021Δsku2 | Deletion mutant of Rm1021 where the 0.5 kb fragment was removed from sku2 | This study |
| Rm1021Δsku3 | Deletion mutant of Rm1021 where the 0.5 kb XhoI fragment was removed from sku3 | This study |
| Rm1021Δsku4 | Deletion mutant of Rm1021 where the 0.6 kb SalI–XhoI fragment was removed from sku4 | This study |
| Rm1021Δsku13 | Rm1021Δsku1 strain whose sku3 was also deleted as in Rm1021Δsku3 | This study |
| Rm1021Δsku134 | Rm1021Δsku1 strain where the 1445 bp XhoI fragment was removed from sku3-sku4 region | This study |
| Rm1021Δsku1234 | Rm1021Δsku134 whose sku2 was also deleted as in Rm1021Δsku2 | This study |
| Rm1021sku2ΔlacZ | Derivative of Rm1021 whose sku2 and 0.6 kb of the downstream ORF was replaced with lacZ | This study |
| Rm1021sku1::pJH104 | Derivative of Rm1021 with a pJH104 insertion in sku1 at 628 bp downstream from the start codon | This study |
| Rm1021sku2::pJH104 | Derivative of Rm1021 with a pJH104 insertion in sku2 at 561 bp downstream from the start codon | This study |
| Rm1021sku3::pJH104 | Derivative of Rm1021 with a pJH104 insertion in sku3 at 649 bp downstream from the start codon | This study |
| Rm1021sku4::pJH104 | Derivative of Rm1021 with a pJH104 insertion in sku4 at 478 bp downstream from the start codon | This study |
| Rm1021ligD1P::pJH104 | Derivative of Rm1021 with a pJH104 insertion in ligD1P at 703 bp downstream from the start codon | This study |
| Rm1021ligD2::pJH104 | Derivative of Rm1021 with a pJH104 insertion in ligD2 at 1003 bp downstream from the start codon | This study |
| Rm1021ligD3::pJH104 | Derivative of Rm1021 with a pJH104 insertion in ligD3 at 1099 bp downstream from the start codon | This study |
| Rm1021ligD4::pJH104 | Derivative of Rm1021 with a pJH104 insertion in ligD4 at 962 bp downstream from the start codon | This study |
| Rm1021sku2-yfpmut2 | Rm1021 derivative where sku2-yfpmut2 replaced the native sku2 | This study |
| Rm1021bacA::pJH104 | Derivative of Rm1021 with a pJH104 insertion in the bacA promoter region without disrupting bacA | G.P. Ferguson (unpublished) |
| Plasmids | ||
| pJH104 | Suicide vector containing a promoterless uidA, Km/NmR | Ferguson et al. (2005) |
| pMP220 | IncP expression vector containing a promoterless lacZ gene, TcR | Spaink et al. (1987) |
| pMP-sku1p | The sku1 promoter region cloned in pMP220 as a 0.5 kb XbaI–PstI fragment | This study |
| pMP-sku2p | The sku2 promoter region cloned in pMP220 as a 0.5 kb XbaI–PstI fragment | This study |
| pMP-sku3p | The sku3 promoter region cloned in pMP220 as a 0.5 kb XbaI–PstI fragment | This study |
| pMP-recAp | The recA promoter region cloned in pMP220 as a 0.5 kb XbaI–PstI fragment | This study |