Fig. 8.
In vivo expression levels of the randomly mutated nopB promoter regions. Mutated nopB promoter regions were fused to promoter-less gfp genes, transferred to NGR234 and NGRΔttsI and assayed after 40-h induction with 2 × 10−6 M apigenin. The results (× 103) are averages of at least three replicate experiments. pPROBE, vector only in NGR234; wt, the wild-type nopB promoter in NGR234; ttsI-, the wild type nopB promoter in NGRΔttsI; the code names refer to randomly mutated nopB promoters.