Fig. 4.

The effect of c-Jun elimination during C2C12 myogenesis. (A) qPCR analysis of endogenous c-Jun mRNA levels prepared and quantitated as described in Fig. 3A. (B) Vimentin CAT constructs -775 and -775 with its tandem AP-1 elements mutated (-775-AP1) were transiently transfected into C2C12 cells and reporter gene activity measured as described in Materials and methods. Mutations in the AP-1 binding sites are indicated as previously described plus the location of surrounding GC-rich elements thought to bind Sp1 are noted in italics (Wu et al., 2007a). (C) ChIP analysis of c-Jun binding over the tandem AP-1 elements in MBs, MTs, and MBs+Ad-ZBP-89 as described in Materials and methods. The calculated fold-value for MBs versus MTs or MBs +Ad-ZBP-89 is shown.