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. 2009 Nov 4;97(9):2567–2576. doi: 10.1016/j.bpj.2009.08.023

Figure 2.

Figure 2

Bis-ANS fluorescence. (A) Representative absolute fluorescence spectra (F(G), cps) when 60 μg/mL multimeric VWF was incubated with varying concentrations of bis-ANS (0–29 μg/mL) for precisely 1 min before fluorescence measurement (excitation λ = 375 nm; emission λ = 420–620 nm). (B) Peak absolute fluorescence intensity (F(G)max) for 60 μg/mL multimeric VWF and 50 μg/mL BSA at various bis-ANS concentrations. (C) Absolute fluorescence spectra when multimeric VWF concentration was varied (0–116.6 μg/mL) in the presence of a fixed amount of bis-ANS (9.7 μg/mL). (D) Peak fluorescence intensity for studies in which multimeric VWF or BSA concentration was varied in the presence of a fixed amount of bis-ANS (9.7 μg/mL for runs with multimeric VWF and 2.4 μg/mL for BSA runs). Arrows in panels B and D indicate the concentrations of bis-ANS and protein, respectively, used in most of the experiments. The sketch at the top of the figure is a schematic of the experimental protocol. Error bars represent the SD for three independent runs.