Figure 4.

Bis-ANS signal reversibility. (A) Fluid shear was applied to 60 μg/mL multimeric VWF using a cone-plate viscometer at 9600/s for 5 min. Bis-ANS was added at various times after shear stoppage (t_add), and fluorescence spectra were measured 1 min thereafter as illustrated in the schematic. Normalized fluorescence (F_c^(G)) data are presented. (B) F_c^(G)_max at a fixed wavelength (484–492 nm for multimeric VWF and 480–482 nm for BSA). Error bars represent the SD for three independent experiments with either multimeric VWF or BSA; ^∗p < 0.05 with respect to the run in which bis-ANS was added 1 min after shear stoppage (i.e., t_add = 1 min).