Table 1.

Peak normal force estimates

	Biomolecule dimensions
Protein name	Sphere radius a1	Separation distance, d	Force coefficient∗, αn	Peak force at G = 6000/s Fn= αnμGa12
BSA	2.3 nm	1 nm	21.03	0.0006 pN
Dimeric VWF	13 nm	94 nm	51.56	0.05 pN
Multimeric VWF (200-mer)	13 nm	94 nm	51.56	11 pN

BSA and dimeric VWF are modeled as dimeric proteins with sphere size a₁ and separation distance d (28). BSA data were taken from previous studies that used small-angle scattering and light scattering (18,39). VWF dimensions are based on electron microscopy (40). Forces applied to multimeric VWF relied on the force calculations of Shankaran and Neelamegham (28), and extrapolations by Zhang et al. (38). Solvent viscosity (μ) is 1 cp.

^∗For details, see Shankaran and Neelamegham (28).