Figure 6.

Effects of single repressive effects of CWO on the period in DD. (a and b) Summary of how the period and peak levels of CLK-CYC and per mRNA react in simulations where the absolute value of a single repressive regulatory weight of CWO is increased (Δ) or decreased (∇) by 10% in DD. Neither CLK-CYC nor the period is affected by similar perturbations of the repressive actions of CWO on tim. (c) Bar graph from an experiment where $\left|{\lambda }_{\text{CWO},per}^{\text{wt}}\right|$ is increased ($\left|{\lambda }_{\text{CWO},per}^{\Delta }\right|$) or decreased ($\left|{\lambda }_{\text{CWO},per}^{\nabla }\right|$) by 10% in DD, leading to higher ($\Delta x_{\text{C/C}}^{\Delta }\left(t_{per}\right)>0$ and $\Delta x_{\text{CWO}}^{\Delta }\left(t_{per}\right)>0$) and lower ($\Delta x_{\text{C/C}}^{\nabla }\left(t_{per}\right)<0$ and $\Delta x_{\text{CWO}}^{\nabla }\left(t_{per}\right)<0$) levels of CLK-CYC and CWO, respectively. The symbol t_per refers to ZT at the peak of per. In the case of ${\lambda }_{\text{CWO},per}^{\Delta }$, the positive transcriptional signal for per outweighs the negative one ($\Delta x_{\text{C/C}}^{\Delta }\left(t_{per}\right){\lambda }_{\text{C/C},per}^{\text{wt}}>\Delta x_{\text{CWO}}^{\Delta }\left(t_{per}\right)\left|{\lambda }_{\text{CWO},per}^{\Delta }\right|$). In the case of ${\lambda }_{\text{CWO},per}^{\nabla }$, the positive transcriptional signal for per is smaller than the negative one ($\Delta x_{\text{C/C}}^{\nabla }\left(t_{per}\right){\lambda }_{\text{C/C},per}^{\text{wt}}<\Delta x_{\text{CWO}}^{\nabla }\left(t_{per}\right)\left|{\lambda }_{\text{CWO},per}^{\nabla }\right|$). (d) Response of the period to the titration of λ_{per, PER}. Notice the apparent linear relation in the interval λ_{per, PER} ∈ [0.0069, 0.0123]; y = 2 × 10^-4 × x + 0.0029 (norm of residuals = 2.7312 × 10^-4). (e) Based on the data plotted in d, and Plots of log(X) versus period, where X denotes the level of CLK-CYC (black), per (blue), or CWO (orange). The arrows in d and e indicate the wt model.