Figure 2.

Correlation between the change in nonpolar surface area introduced by the mutation ($\Delta {\text{ASA}}_{\text{Mut}/\text{WT}}^{\text{U}-\text{F}}$) and the change in stability of the mutants ($\Delta \Delta G_{\text{Mut}/\text{WT}}^{0,\text{U}-\text{F}}$) for (A) the K-to-Q mutant set only and (B) all mutant types considered: K-to-Q (solid circles), K-to-A (star), and D-to-E (open squares). Error bars in $\Delta {\text{ASA}}_{\text{Mut}/\text{WT}}^{\text{U}-\text{F}}$ arise from the values calculated for the 12 conformations in the manifold of the solution NMR structure and for the two differently modeled structures (K41Q and K7Q). R² corresponds to the coefficient of determination.