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. 2009 Aug 25;37(19):6635–6643. doi: 10.1093/nar/gkp615

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© The Author 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Determination of the first-order rate constants for the deamination of 5-methylcytosine glycol in a 27-mer duplex DNA. Plotted are the ratios of peak area found in the SIC for the m/z 684 →541 transition (for pTgpdG) over that found in the SIC for monitoring the fragmentation of the ion of m/z 330 (for pdA) versus the deamination time. The solid square and triangle represent the results based on the formation of pTgpdG containing the (5R,6S) (the 19.5-min peak in Figure 1d) and (5S,6R) (the 20.1-min peak in Figure 1d) diastereomers of Tg (Scheme 1), respectively. The deamination reaction conditions were described in ‘Materials and Methods’ section.