Table 1.
Primer sequences and formulae
| mRNA | PCR primers | Sequencing primer | Sequences of each isoform | Nucleotide dispensation order | Formulae |
|---|---|---|---|---|---|
| α-tropo, 6A/6B | b-fwd CAGAGGAACGTGCTGAACTTT | s TCCTTCTGGGAGTACTT | 6A: CTCTTCTG | ACTCTCGTGC | (6B)%=100 × G7/(G7 + G9) |
| rev ATTTGTCCTCCTTCTGGGAGTA | 6B: CTCTGCC | (6A)%=100 × G9/(G7 + G9) | |||
| α-tropo, | Fwd AGGCTGAAACACGTGCTGAG | s1 GTCCATTGATGATTTAGA | 9A:AGATGA | CAGAGTGA | (O5)%=100 × G5/(G5 + G7) |
| terminal exons | b-rev CGCCAGGGTTTTCCCAGTCACGAC | O5:AGAGAAA | (9A)%=100 × G7/(G5 + G7) | ||
| s2 CTCTCAATGACATGACTTC | 9′ :AATGTAAATTC | GATGATATCG | (9′)%=100 × G4/(G4 + G10) | ||
| 9B:AATATAAAATG | (9B)%=100 × G10/(G4 + G10) | ||||
| Tnnt2 | fwd GGAGAGCCGAGAGCATGT | s1 GGAGTACGAGGAGGAAC | 4 and 9: AGGAAGAAGCTG | GAGAGAGAGCTGAG | (4 + 9)%=100 × G12/(G9 + G12 + G14) |
| b-rev TCAGGACCAACCTCTTCTACG | 5: AGGAAGAGGAAGA | (5)%=100 × G9/(G9 + G12 + G14) | |||
| ref: AGGAAGAGCAAG | (ref)%=100 × G14/(G9 + G12 + G14) | ||||
| s2 AACAGGAAGAAGCTGTG | 4: GAAGAGCAAGA | TGAGAGCAGAC | (4)%=100 × C7/(C7 + C11) | ||
| 9: GAAGAGGAAGAC | (9)%=100 × C11/(C7 + C11) |
b, biotinylated; fwd, forward primer; rev, reverse primer; s, sequencing primer.