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Assay miniaturization to 1536-well format. Time course assays for pol β (A), pol η (B), pol ι (C) and pol κ (D) were conducted in 4-μl reaction volumes with 10 nM of enzyme and 50 nM substrate 0–10. Filled and open symbols represent the presence or absence of enzyme, respectively. Averages and standard deviations shown are from 64 wells per reaction condition.
