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. 2009 Sep 16;37(19):6291–6304. doi: 10.1093/nar/gkp659

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Number of primers required to amplify fragments from each complete genome or segment in the family versus the number of available sequences in that family. Primer parameters were length 17–21-mers, T_m between 55°C and 60°C.