Table 1.

Primer counts for viral family primer sets, as described in the methods

Grey shaded entries indicate where calculations were not run to completion. In other cases (not shaded) where fewer than 100% of targets were predicted to be amplified, the algorithm failed to find primer pairs that met all the required specifications for the remaining targets, that is, primers in the right length, T_m, and amplicon length range, with hairpin and dimer avoidance with other primers already selected to be in the set, could not be found.

For 10–15-mers, T_m = 40–45°C and for 17–21-mers, T_m = 55–60°C.

For both, the amplicon length range was 200–800 bp.

None of the calculations that completed required more than the 16 GB of RAM that was available.