Table 4.
Sequence and size (nt) information for the 16 primers in the predicted family-level multiplex for Poxviridae viruses used in these experiments
| Primer sequence | Primer size (nt) |
|---|---|
| CGGAGACCAA (FP 617) | 10 |
| TCGTCGTCCA (RP 617) | 10 |
| TGTTGGTGTG | 10 |
| TCGTCTACGA | 10 |
| TGTGGTCCTT | 10 |
| CCATGTTCGC | 10 |
| TCGAGGAGAA | 10 |
| CCGGCTCCAG | 10 |
| TGAACCTGGT | 10 |
| GCGCACGTAC | 10 |
| TCACGCATCT | 10 |
| GGGAAACAGC | 10 |
| ACCGTTGTCA | 10 |
| TACCATCGTC | 10 |
| AACCTGTGCA | 10 |
| GGCGGAGGTA | 10 |
The forward and reverse primers (FP, RP) in bold are predicted to amplify vaccinia Lister with the indicated product length (617-bp amplicon size).