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Fast cycling PCR. Mean Cq values and standard deviations (error bars) from quadruplicate reactions carried out with ZNA-E7 (black bars), DNA-E7 (white bars) or LNA-E7 (grey bars) primers as a function of annealing/extension time. Reactions were carried out on 10 ng of target genomic DNA with 100 nM each primer using Sensimix NoRef DNA kit (Quantace) 0.5× concentrated. MgCl2 was 1.5 mM (ZNA primers) and 3 mM (LNA and DNA primers). Cycling conditions were 95°C (10 s), 60°C as indicated.
