Table 1.

Sequence information

Gene	Primer	5′-3′ sequences			Position	NCBI Accession number
HPV16-E7			% GC	Tma		NM001526
	DNA-E7F	GAG GAG GAG GAT GAA ATA GAT GGT	45.8	62.1°C	658–681
	DNA-E7R	GCC CAT TAA CAG GTC TTC CAA	47.8	61.3°C	816–796
	ZNA-E7F	Z4-GAG GAG GAG GAT GAA ATA GAT GGT
	ZNA-E7R	Z4-GCC CAT TAA CAG GTC TTC CAA
	LNA-E7F	G+AG GA+G GAG GAT GAA ATA GAT GGT
	LNA-E7R	GC+C CAT +TAA CAG GTC TTC CAA
HPV16-L1
	DNA-L1F	TTT GTT ACT GTT GTT GAT ACT AC	30.4	56.4°C	6624–6646
	DNA-L1R	GAA AAA TAA ACT GTA AAT CAT ATT C	20.0	53.9°C	6741–6765
	ZNA-L1F	Zn-TTT GTT ACT GTT GTT GAT ACT AC
	ZNA-L1R	Zn-GAA AAA TAA ACT GTA AAT CAT ATT C
	LNA-L1F	TT+T GTT +ACT GTT GTT GAT ACT AC
	LNA-L1R	GA+A AAA +TAA ACT GTA AAT CAT ATT C
HPRT1			Localization			NM000194
	HPRT1F	TCCTTGGTCAGGCAGTATAATCCA	Exon6		527–550
	HPRT1R	GTCTGGCTTATATCCAACACTTCGT	Exon7		580–566
	HPRT1probe	FAM-CACCAGCAAGCTTGC-MGBnfq	Junction 6–7		613–592
	ZNA-HPRT1R	Z4-GTCTGGCTTATATCCAACACTTCGT
HMGA2 (variant 1)						NM003483
	HMGA2F	CTAGGAAATGGCCACAACAAGTTG	Exon4		1050–1073
	HMGA2R	GGCAGACTCTTGTGAGGATGT	Exon5		1129–1109
	HMGA2probe	FAM-CTGCTCAGGAGGAAAC-MGBnfq	Junction 4–5		1086–1101
	ZNA-HMGA2R	Z4-CTAGGAAATGGCCACAACAAGTTG

Z_n denotes n spermine units, +N denotes LNA nucleotide.

^aT_m prediction from IDT SciTools web server for 100 nM oligonucleotide and 3 mM MgCl₂. At 1.5 mM MgCl₂, the T_m is predicted to be reduced by 2°C.