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. 2009 Aug 20;37(19):e130. doi: 10.1093/nar/gkp661

Table 4.

PCR efficiencies (E) calculated from the slope of standard curves using ZNA-E7, DNA-E7 and LNA-E7 primers

Primers Cqa E R2 b
ZNA-E7 17.7 ∓ 0.01 0.94 0.999
DNA-E7 20.7 ∓ 0.09 0.96 0.993
LNA-E7 19.3 ∓ 0.00 0.89 0.999

Target genomic DNA (10 ng) and 10-fold serial dilutions (up to 0.01 ng) spiked in 10 ng of control DNA were amplified in duplicate with 100 nM each primer using QuantiFast SYBR Green PCR kit (Qiagen). Cycling conditions were 95°C (10 s), 60°C (30 s).

aCq obtained from amplification of 10 ng target DNA.

bFrom the linear regression.