Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 2;37(19):6378–6388. doi: 10.1093/nar/gkp678

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Schematic of recipient (A) and donor (B) plasmids. (A) Psoralen ICL sites are at the same position in all plasmids, the dashed line indicates the purine-rich strand bound by pTFO1. This TFO binding sequence was inserted in the plasmids via an insertion of 73 bp shown in (C). (B) The donor plasmid, p1.2kHD, has a 1.2 kb fragment homologous to the recipient plasmids, but contains the wild-type supF gene. p1.2kHD has two restriction enzyme sites for BsaAI and Bsu36I, which are absent from the recipient plasmids. (C) The insertion sequence containing the TFO1 binding sequence. The unique SalI site is used to confirm the presence of the ICL, and the unique BglII site is used to confirm the presence of the triplex structure formed by TFO binding.