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. 2009 Aug 31;37(19):6477–6490. doi: 10.1093/nar/gkp681

Figure 4.

Figure 4.

Involvement of exon 7A sequence in splicing regulation by MBNL1. (A) Response of truncated Clcn1 minigenes to MBNL1 is dependent on exon 7A. Structures of 6-7A, 7A-7 and 6/7 deletion mutants are indicated in the upper left. Splicing analysis of these mutants shows both spliced and unspliced products as indicated. The bar chart shows the quantified ratio of spliced products. (B) Deletion in the 3′ region of exon 7A did not abolish the response to MBNL1. The structure of the 6-7AΔ mutant is shown (left). Splicing products of 6-7AΔ and quantification are shown as in (A). (C) Splicing regulation of the 7A/(−52)7A minigene. 7A/(−52)7A was made from 7A-7 by replacing exon 7 and its upstream 52 nt with the corresponding region of exon 7A (left). Splicing of 7A/(−52)7A was responsive to MBNL1 (right). Lanes ‘V’ and ‘M’ indicate co-transfection with the empty vector and MBNL1, respectively. Bars represent the ratio of spliced bands. Statistical analysis was performed by two-tailed t-test in comparison with the empty vector. *P < 0.05 from three independent experiments.