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. 2009 Sep 2;37(19):6550–6561. doi: 10.1093/nar/gkp693

Figure 5.

Figure 5.

The changes in mRNA stability and gene expression profiles of AMPs after Tis11 knockdown. (A and B) S2* cells were transfected with dsRNA specific for Tis11 or EGFP and cultured for 72 h. After exposure of cells to LPS for 2 h, Act.D was added and the preparation incubated for the indicated times. The qRT–PCR was performed to detect CecA1 (A) and Dpt (B) mRNA remaining at each time point, taking the expression level at the time of Act.D addition as 1. (**P<0.01 for EGFP dsRNA versus Tis11 dsRNA at each time point) (C and D) The qRT–PCR analysis of CecA1 (C) or Dpt (D) transcripts in S2* cells after LPS treatment under EGFP or Tis11 RNAi conditions. The fold change of CecA1 or Dpt mRNA was analyzed, taking the expression level in cells transfected with EGFP dsRNA at time point of 0 h as 1. The data presented are mean ± SD of four independent experiments.