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. 2009 Sep 2;37(19):6550–6561. doi: 10.1093/nar/gkp693

Figure 7.

Figure 7.

Effect of p38MAPK inhibition by SB203580 on AMP mRNA stability and Tis11 expression. SB203580 or vehicle (1% DMSO) pretreated S2* cells were incubated with 10 μg/ml LPS for 2 h. Act.D chase studies were performed to detect CecA1 (A) and Dpt (B) mRNA remaining at each time point, taking the expression level at the time of Act.D addition as 1. (C) S2* cells were pretreated for 0.5 h with SB203580 or vehicle. Afterward, cells were incubated with LPS for different times. The fold changes of Tis11 mRNA were detected at each time point, taking the mRNA level of vehicle at 0 h as 1. (D) S2* cells were transfected with dsRNA specific for Tis11 or EGFP and cultured for 72 h. The cells were then treated as described in (A). qRT–PCR was performed to detect CecA1 mRNA remaining at each time point, taking the expression level at the time of Act.D addition as 1.