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. 2009 Sep 2;37(19):6389–6399. doi: 10.1093/nar/gkp695

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — HMO2 binds preferentially to supercoiled DNA compared to linear DNA. (A) Reactions with 5 fmol of 50 bp looped DNA and 4 µM HMO2. Lane 1, DNA only, lanes 2, 50 bp looped DNA and 4 µM HMO2. Lanes 3–11 with 0.5–50 fmol supercoiled pGEM5. (B) Titration with linear pGEM5 with 5′ overhangs.