Table 1.

MtDNA depletion in cells expressing topoisomerase I constructs

Constructs	MtDNA copies (±SEM)	Number of clones investigated	P-value
MY	1.0 ± 0.33	13	–
MY-Top1mt	0.94 ± 0.25	27	–
MY-Top1	7.23 × 10−5 ± 5.56 × 10−5a	2	<0.0001
MY-Top1191–765	1.97 × 10−4 ± 3.0 × 10−4a	3	<0.0001
MY-Top1191–765*	1.19 × 10−4 ± 2.59 × 10−5a	3	<0.0001

MtDNA copy numbers were determined by quantitative real time PCR.

^aValues differed significantly from the control (MY) according to an unpaired t-test. Values of control cells (MY) were arbitrarily set to 1.0.