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. 2009 Sep 4;37(19):6503–6514. doi: 10.1093/nar/gkp711

Figure 3.

Figure 3.

RNA from wild-type DbpA 50S subunits, DbpA R331A 50S subunits, DbpA R331A 45S particles, RrmJ 50S subunits, ΔrrmJ 50S subunits and ΔrrmJ 40S particles was analyzed by primer extension using 5′-end labeled primers 1 and 2. (A) The positions of the mature 5′-end (M) of 23S rRNA and the longer products (+3 and +7) of pre-23S rRNA are as indicated. (B) Primer 2 is complementary to nucleotides 3′ of hairpin 92 of 23S rRNA and permits detection of the 2′-O-methyl at U2552 by reverse transcription in the presence of low dNTP concentration. H and L indicate high (1 mM) and low (4 µM) dNTP concentrations. Primer extension stops at modification sites are seen as bands in the low dNTP lanes. On both gels, the first four lanes show a sequencing ladder obtained using the same primers and 23S rRNA transcript. Control experiments were performed with 23S rRNA transcript and 23S + 16S rRNA purified from MRE600 cells.