Table 2.

ATP hydrolysis rates of rRNA and particles

Strain	Substrate	ATP hydrolyzed per DbpA (min−1)	+40 nM R331A	+100 nM R331A	+200 nM R331A
	ATP only	0.05 ± 0.01
	23S rRNA	25.1 ± 0.9	18.6 ± 1.1	10.4 ± 1.2	4.6 ± 1.0
Tuner + dbpA	50S	0.4 ± 0.1
Tuner + R331A	45S	10.6 ± 1.5	7.3 ± 1.3	5.0 ± 0.4	4.0 ± 0.8
	50S	2.1 ± 0.2
rrmJ	50S	0.5 ± 0.2
ΔrrmJ	40S	10.3 ± 1.4
	50S	5.1 ± 1.5
LiCl cores	0.4M	4.5 ± 0.6
	0.6M	15.3 ± 2.0
	0.8M	19.3 ± 1.4

ATP hydrolyzed per DbpA was monitored over time in the presence of 100 nM 23S rRNA or ribosome particles, 40 nM DbpA and 20 μM ATP in buffer (50 mM Hepes pH 7.5, 20 mM KC1, 5 mM MgCl₂, 1 mM DTT, 0.1% Tween 20) at 24°C.