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. 2009 Sep 3;37(19):6587–6599. doi: 10.1093/nar/gkp714

Figure 4.

Figure 4.

Comparably expressed mutant forms of sh-B971 do not induce IFN. (A) IFN activation by select sh-B971 mutants. The shRNA expression plasmids were transfected into 293FT cells with the luciferase reporters to measure IFN-β promoter activation. The firefly luciferase readings were normalized to Renilla luciferase readings, and the value of sh-B971 was set to 100. (B) Intracellular levels of siRNA products of sh-B971 and mutants. RNA was extracted from transfected 293FT cells and analyzed by northern blotting with a DNA oligonucleotide probe that is complementary to all three forms of sh-B971 (wt, sh-A1/G and Loop A).