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. 2009 Nov;128(3):324–333. doi: 10.1111/j.1365-2567.2009.03130.x

Figure 2.

Figure 2

Invariant natural killer T (iNKT) cell lines produce cytokines after α-galactosylceramide (αGalCer) stimulation. iNKT cell lines (AC6, AC8 and AC10) stimulated with αGalCer more than 2 weeks earlier were washed and re-suspended with complete RPMI medium without cytokines. (a) 1 × 105 iNKT cells were incubated with 1 × 105 bone marrow dendritic cells (BMDCs) and αGalCer (100 ng/ml). Supernatants were collected and cytokine production was measured by enzyme-linked immunosorbent assay (ELISA). (b) 1 × 105 iNKT cells were incubated with 1 × 105 wild-type (WT) BMDCs or CD1d−/− BMDCs and αGalCer. The interferon (IFN)-γ content in culture supernatants was determined by ELISA. Results are representative of two separate experiments.