Figure 5.
Invariant natural killer T (iNKT) cell lines respond to a microbial antigen and produce cytokines. 1 × 105 NKT cells were incubated with the indicated concentrations of glycosphingolipid (GSL)-1 or α-galactosylceramide (αGalCer; 10 ng/ml) in the presence of 1 × 105 wild-type (WT) BMDCs or CD1d−/− BMDCs. Supernatants were collected to determine interferon (IFN)-γ and interleukin (IL)-4 production by enzyme-linked immunosorbent assay (ELISA). Results are representative of two separate experiments.