Table 1.

Vβ usage of spleen Invariant natural killer T (iNKT) cells and iNKT cell lines

	Vβ8.1/8.2	Vβ14	Vβ10	Vβ6	Vβ7	Vβ2	Vβ12
Spleen iNKT cell	34·3 ± 3·7	0·8 ± 0·9	2·0 ± 0·8	0·8 ± 0·4	1·9 ± 0·3	0·2 ± 0·0	1·1 ± 0·5
iNKT cell line	32·8 ± 9·5	32·6 ± 23·8*	3·7 ± 1·9	3·4 ± 1·3*	3·8 ± 1·4*	1·0 ± 0·4*	0·6 ± 0·2

Splenocytes of Vα14Tg mice were stained with anti-CD19 monoclonal antibody (mAb), anti-CD3e mAb, CD1d-tetramer and each anti-Vβ mAb. iNKT cells were identified as CD19^negative CD3^positive CD1d-tetramer ^positive cells. iNKT cell lines were stained with a panel of anti-Vβ mAbs. The frequencies of cells expressing the indicated Vβ chains among iNKT cells are expressed as an average percentage ± standard deviation. n = 3–7 per group.

^*< 0·05, Welche’s t-test.