Figure 7.

Splenectomy reduced the antibody production to damaged tissue. Wounded tissues were taken from sham-operated or splenectomized 11-week-old C57BL/6 mice at 6–96 hr after punch biopsy. Tissue lysates/homogenates were subjected to sodium dodecyl sulphate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membrane. Sera (diluted 1 : 100) taken after 1 week from sham-operated or splenectomized mice (10 weeks old) were reacted with membranes as a primary antibody and then membranes were reacted with anti-mouse immunoglobulin G1 (IgG1) secondary antibodies. Bound antibodies were detected using the enhanced chemiluminescence system. (a) Sera were taken from sham-operated mice. Tissues were taken at 0 hr (before punch biopsy) and 6 hr after punch biopsy. (b) Sera were taken from sham-operated mice. Tissues were taken at 6, 24, 48 or 96 hr after punch biopsy from sham-operated or splenectomized mice, (c) Sera were taken from splenectomized mice. Tissues were taken at 6, 24, 48 or 96 hr after punch biopsy from sham-operated or splenectomized mice.