Table 1.
Immunoprecipitated bands (Fig. 8) were cut and analysed by LC/MS/MS
| (a) < myosin, heavy polypeptide 9, non-muscle isoform 1 –Mus musculus; 226 000 > | |
| 1 | MAQQAADKYL YVDKNFINNP LAQADWAAKK LVWVPSSKNG FEPASLKEEV |
| 51 | GEEAIVELVE NGKKVKVNKD DIQKMNPPKF SKVEDMAELT CLNEASVLHN |
| 101 | LKERYYSGLI YTYSGLFCVV INPYKNLPIY SEEIVEMYKG KKRHEMPPHI |
| 151 | YAITDTAYRS MMQDREDQSI LCTGESGAGK TENTKKVIQY LAHVASSHKS |
| 201 | KKDQGELERQ LLQANPILEA FGNAKTVKND NSSRFGKFIR INFDVNGYIV |
| 251 | GANIETYLLE KSRAIRQAKE ERTFHIFYYL LSGAGEHLKT DLLLEPYNKY |
| 301 | RFLSNGHVTI PGQQDKDMFQ ETMEAMRIMG IPEDEQMGLL RVISGVLQLG |
| 351 | NIAFKKERNT DQASMPDNTA AQKVSHLLGI NVTDFTRGIL TPRIKVGRDY |
| 401 | VQKAQTKEQA DFAIEALAKA TYERMFRWLV LRINKALDKT KRQGASFIGI |
| 451 | LDIAGFEIFD LNSFEQLCIN YTNEKLQQLF NHTMFILEQE EYQREGIEWN |
| 501 | FIDFGLDLQP CIDLIEKPAG PPGILALLDE ECWFPKATDK SFVEKVVQEQ |
| 551 | GTHPKFQKPK QLKDKADFCI IHYAGKVDYK ADEWLMKNMD PLNDNIATLL |
| 601 | HQSSDKFVSE LWKDVDRIIG LDQVAGMSET ALPGAFKTRK GMFRTVGQLY |
| 651 | KEQLAKLMAT LRNTNPNFVR CIIPNHEKKA GKLDPHLVLD QLRCNGVLEG |
| 701 | IRICRQGFPN RVVFQEFRQR YEILTPNSIP KGFMDGKQAC VLMIKALELD |
| 751 | SNLYRIGQSK VFFRAGVLAH LEEERDLKIT DVIIGFQACC RGYLARKAFA |
| 801 | KRQQQLTAMK VLQRNCAAYL RLRNWQWWRL FTKVKPLLNS IRHEDELLAK |
| 851 | EAELTKVREK HLAAENRLTE METMQSQLMA EKLQLQEQLQ AETELCAEAE |
| 901 | ELRARLTAKK QELEEICHDL EARVEEEEER CQYLQAEKKK MQQNIQELEE |
| 951 | QLEEEESARQ KLQLEKVTTE AKLKKLEEDQ IIMEDQNCKL AKEKKLLEDR |
| 1001 | VAEFTTNLME EEEKSKSLAK LKNKHEAMIT DLEERLRREE KQRQELEKTR |
| 1051 | RKLEGDSTDL SDQIAELQAQ IAELKMQLAK KEEELQAALA RVEEEAAQKN |
| 1101 | MALKKIRELE TQISELQEDL ESERASRNKA EKQKRDLGEE LEALKTELED |
| 1151 | TLDSTAAQQE LRSKREQEVS ILKKTLEDEA KTHEAQIQEM RQKHSQAVEE |
| 1201 | LADQLEQTKR VKATLEKAKQ TLENERGELA NEVKALLQGK GDSEHKRKKV |
| 1251 | EAQLQELQVK FSEGERVRTE LADKVTKLQV ELDSVTGLLS QSDSKSSKLT |
| 1301 | KDFSALESQL QDTQELLQEE NRQKLSLSTK LKQMEDEKNS FREQLEEEEE |
| 1351 | AKRNLEKQIA TLHAQVTDMK KKMEDGVGCL ETAEEAKRRL QKDLEGLSQR |
| 1401 | LEEKVAAYDK LEKTKTRLQQ ELDDLLVDLD HQRQSVSNLE KKQKKFDQLL |
| 1451 | AEEKTISAKY AEERDRAEAE AREKETKALS LARALEEAME QKAELERLNK |
| 1501 | QFRTEMEDLM SSKDDVGKSV HELEKSKRAL EQQVEEMKTQ LEELEDELQA |
| 1551 | TEDAKLRLEV NLQAMKAQFE RDLQGRDEQS EEKKKQLVRQ VREMEAELED |
| 1601 | ERKQRSMAMA ARKKLEMDLK DLEAHIDTAN KNREEAIKQL RKLQAQMKDC |
| 1651 | MRELDDTRAS REEILAQAKE NEKKLKSMEA EMIQLQEELA AAERAKRQAQ |
| 1701 | QERDELADEI ANSSGKGALA LEEKRRLEAR IAQLEEELEE EQGNTELIND |
| 1751 | RLKKANLQID QINTDLNLER SHAQKNENAR QQLERQNKEL KAKLQEMESA |
| 1801 | VKSKYKASIA ALEAKIAQLE EQLDNETKER QAASKQVRRT EKKLKDVLLQ |
| 1851 | VEDERRNAEQ FKDQADKAST RLKQLKRQLE EAEEEAQRAN ASRRKLQREL |
| 1901 | EDATETADAM NREVSSLKNK LRRGDLPFVV TRRIVRKGTG DCSDEEVDGK |
| 1951 | ADGADAKAAE |
| (b) < Carbamoyl-phosphate synthase [ammonia], mitochondrial precursor –Mus musculus 164 000 > | |
| 1 | MTRILTACKV VKTLKSGFGF ANVTTKRQWD FSRPGIRLLS VKAKTAHIVL |
| 51 | EDGTKMKGYS FGHPSSVAGE VVFNTGLGGY PEALTDPAYK GQILTMANPI |
| 101 | IGNGGAPDTT ARDELGLNKY MESDGIKVAG LLVLNYSNDY NHWLATKSLG |
| 151 | QWLQEEKVPA IYGVDTRMLT KIIRDKGTML GKIEFEGQSV DFVDPNKQNL |
| 201 | IAEVSTKDVK VFGKGNPTKV VAVDCGIKNN VIRLLVKRGA EVHLVPWNHD |
| 251 | FTQMEYDGLL IAGGPGNPAL AQPLIQNVKK ILESDRKEPL FGISTGNIIT |
| 301 | GLAAGAKSYK MSMANRGQNQ PVLNITNRQA FITAQNHGYA LDNTLPAGWK |
| 351 | PLFVNVNDQT NEGIMHESKP FFAVQFHPEV SPGPTDTEYL FDSFFSLIKK |
| 401 | GKGTTITSVL PKPALVASRV EVSKVLILGS GGLSIGQAGE FDYSGSQAVK |
| 451 | AMKEENVKTV LMNPNIASVQ TNEVGLKQAD AVYFLPITPQ FVTEVIKAER |
| 501 | PDGLILGMGG QTALNCGVEL FKRGVLKEYG VKVLGTSVES IMATEDRQLF |
| 551 | SDKLNEINEK IAPSFAVESM EDALKAADTI GYPVMIRSAY ALGGLGSGIC |
| 601 | PNKETLIDLG TKAFAMTNQI LVERSVTGWK EIEYEVVRDA DDNCVTVCNM |
| 651 | ENVDAMGVHT GDSVVVAPAQ TLSNAEFQML RRTSVNVVRH LGIVGECNIQ |
| 701 | FALHPTSMEY CIIEVNARLS RSSALASKAT GYPLAFIAAK IALGIPLPEI |
| 751 | KNVVSGKTSA CFEPSLDYMV TKIPRWDLDR FHGTSSRIGS SMKSVGEVMA |
| 801 | IGRTFEESFQ KALRMCHPSV DGFTPRLPMN KEWPANLDLK KELSEPSSTR |
| 851 | IYAIAKALEN NMSLDEIVRL TSIDKWFLYK MRDILNMDKT LKGLNSDSVT |
| 901 | EETLRKAKEI GFSDKQISKC LGLTEAQTRE LRLKKNIHPW VKQIDTLAAE |
| 951 | YPSVTNYLYV TYNGQEHDIK FDEHGIMVLG CGPYHIGSSV EFDWCAVSSI |
| 1001 | RTLRQLGKKT VVVNCNPETV STDFDECDKL YFEELSLERI LDIYHQEACN |
| 1051 | GCIISVGGQI PNNLAVPLYK NGVKIMGTSP LQIDRAEDRS IFSAVLDELK |
| 1101 | VAQAPWKAVN TLNEALEFAN SVGYPCLLRP SYVLSGSAMN VVFSEDEMKR |
| 1151 | FLEEATRVSQ EHPVVLTKFV EGAREVEMDA VGKEGRVISH AISEHVEDAG |
| 1201 | VHSGDATLML PTQTISQGAI EKVKDATRKI AKAFAISGPF NVQFLVKGND |
| 1251 | VLVIECNLRA SRSFPFVSKT LGVDFIDVAT KVMIGESIDE KRLPTLEQPI |
| 1301 | IPSDYVAIKA PMFSWPRLRD ADPILRCEMA STGEVACFGE GIHTAFLKAM |
| 1351 | LSTGFKIPQK GILIGIQQSF RPRFLGVAEQ LHNEGFKLFA TEATSDWLNA |
| 1401 | NNVPATPVAW PSQEGQNPSL SSIRKLIRDG SIDLVINLPN NNTKFVHDNY |
| 1451 | VIRRTAVDSG IALLTNFQVT KLFAEAVQKS RTVDSKSLFH YRQYSAGKAA |
| (c) < Actin –Mus musculus 42 000 > | |
| 1 | MCDDEETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG |
| 51 | QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP |
| 101 | EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT |
| 151 | TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY |
| 201 | SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI |
| 251 | TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV |
| 301 | LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS |
| 351 | LSTFQQMWIS KQEYDEAGPS IVHRKCF |
| (c) < Argininosuccinate synthase –Mus musculus 46 000 > | |
| 1 | MKWVTFLLLL FVSGSAFSRG VFRREAHKSE IAHRYNDLGE QHFKGLVLIA |
| 51 | FSQYLQKCSY DEHAKLVQEV TDFAKTCVAD ESAANCDKSL HTLFGDKLCA |
| 101 | IPNLRENYGE LADCCTKQEP ERNECFLQHK DDNPSLPPFE RPEAEAMCTS |
| 151 | FKENPTTFMG HYLHEVARRH PYFYAPELLY YAEQYNEILT QCCAEADKES |
| 201 | CLTPKLDGVK EKALVSSVRQ RMKCSSMQKF GERAFKAWAV ARLSQTFPNA |
| 251 | DFAEITKLAT DLTKVNKECC HGDLLECADD RAELAKYMCE NQATISSKLQ |
| 301 | TCCDKPLLKK AHCLSEVEHD TMPADLPAIA ADFVEDQEVC KNYAEAKDVF |
| 351 | LGTFLYEYSR RHPDYSVSLL LRLAKKYEAT LEKCCAEANP PACYGTVLAE |
| 401 | FQPLVEEPKN LVKTNCDLYE KLGEYGFQNA ILVRYTQKAP QVSTPTLVEA |
| 451 | ARNLGRVGTK CCTLPEDQRL PCVEDYLSAI LNRVCLLHEK TPVSEHVTKC |
| 501 | CSGSLVERRP CFSALTVDET YVPKEFKAET FTFHSDICTL PEKEKQIKKQ |
| 551 | TALAELVKHK PKATAEQLKT VMDDFAQFLD TCCKAADKDT CFSTEGPNLV |
| 601 | TRCKDALA |
| (c) < α-actinin-4 –Mus musculus 104 000 > | |
| 1 | MVDYHAANQA YQYGPNSGGG NGAGGGGSMG DYMAQEDDWD RDLLLDPAWE |
| 51 | KQQRKTFTAW CNSHLRKAGT QIENIDEDFR DGLKLMLLLE VISGERLPKP |
| 101 | ERGKMRVHKI NNVNKALDFI ASKGVKLVSI GAEEIVDGNA KMTLGMIWTI |
| 151 | ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNVQNFH ISWKDGLAFN |
| 201 | ALIHRHRPEL IEYDKLRKDD PVTNLNNAFE VAEKYLDIPK MLDAEDIVNT |
| 251 | ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEHLMEDYE |
| 301 | RLASDLLEWI RRTIPWLEDR VPQKTIQEMQ QKLEDFRDYR RVHKPPKVQE |
| 351 | KCQLEINFNT LQTKLRLSNR PAFMPSEGRM VSDINNGWQH LEQAEKGYEE |
| 401 | WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLKQR DYETATLSDI |
| 451 | KALIRKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSH NVNTRCQKIC |
| 501 | DQWDNLGSLT HSRREALEKT EKQLETIDQL HLEYAKRAAP FNNWMESAME |
| 551 | DLQDMFIVHT IEEIEGLISA HDQFKSTLPD ADREREAILA IHKEAQRIAE |
| 601 | SNHIKLSGSN PYTTVTPQII NSKWEKVQQL VPKRDHALLE EQSKQQSNEH |
| 651 | LRRQFASQAN MVGPWIQTKM EEIGRISIEM NGTLEDQLSH LKQYERSIVD |
| 701 | YKPSLDLLEQ QHQLIQEALI FDNKHTNYTM EHIRVGWEQL LTTIARTINE |
| 751 | VENQILTRDA KGISQEQMQE FRASFNHFDK DHGGALGPEE FKACLISLGY |
| 801 | DVENDRQGDA EFNRIMSVVD PNHSGLVTFQ AFIDFMSRET TDTDTADQVI |
| 851 | ASFKVLAGDK NFITAEELRR ELPPDQAEYC IARMAPYQGP DAAPGALDYK |
| 901 | SFSTALYGES DL |
Upper band (220 000∼250 000) was matched with myosin, heavy polypeptide 9, non-muscle isoform 1 (226 000). Lower band (42 000∼50 000) was matched with actin, α-cardiac muscle, 42 000 actin, cytoplasmic 1 –Mus musculus 42 000 argininosuccinate synthase; α-actinin-4 –Mus musculus 104 000, argininosuccinate synthase –Mus musculus (Mouse) 46 000. Bold letters indicate the fit regions of LS/MS analysis