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. 2009 Nov;128(3):393–404. doi: 10.1111/j.1365-2567.2009.03121.x

Figure 4.

Figure 4

Adenosine deaminase (ADA) increases cytokine secretion in cultures of anti-CD3-triggered T cells from human immunodeficiency virus 1 (HIV-1)-infected patients. T cells from HIV-1-infected patients were stimulated with 1 ng/ml anti-CD3 in the absence or presence of 4 μm ADA and cytokine levels were determined at days 2 and 6 (a) or day 2 (b) in culture supernatants as indicated in the Materials and methods. (a) Cytokine production when cells were stimulated with anti-CD3 in the absence (black columns) or presence of 4 μm ADA (white columns); data from one representative experiment of five are shown. Data are the mean ± standard deviation of triplicates. (b) Cytokine production in culture supernatants of anti-CD3-triggered T cells from five HIV-1-infected individuals in the absence of ADA (anti-CD3) or in the presence of 4 μm ADA (anti-CD3 + ADA). Values are expressed as in-folds, calculated as the ratio of the cytokine concentration (pg/ml) produced by stimulated T cells versus that produced by non-stimulated T cells. Each symbol represents an HIV-1-infected individual. Data are the mean ± standard deviation of triplicates. For each donor, IL-2 and IL-4 production was also analysed, and was < 20 pg/ml in the case of IL-4 and < 10 pg/ml for IL-2.