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. 2009 Nov;128(3):439–450. doi: 10.1111/j.1365-2567.2009.03134.x

Figure 2.

Figure 2

Effect of human recombinant prostaglandin E2 (PGE2) and transforming growth factor-β (TGF-β) on cytosine–phosphate–guanosine oligodesoxynucleotide (CpG-ODN)-stimulated interferon-α (IFN-α), TNF and interleukin-8 (IL-8) production in plasmacytoid dendritic cells (PDC). PDC were pre-incubated with PGE2 and TGF-β before stimulation with toll-like receptor (TLR). After 24 hr, the production of IFN-α, TNF and IL-8 production was measured using enzyme-linked immunosorbent assays (ELISAs). Black bars show unstimulated conditions; grey (CpG alone), white (TGF + CpG) and striped (PGE2 + CpG or TGF + PGE2 + CpG) bars show values after stimulation with CpG ODN 2006, respectively. The mean values ± standard error of the mean (SEM) of four individual donors are shown. (a) IFN-α. P*[CpG/TGF + CpG] = 0·007; P*[CpG/PGE2 1 μm+ CpG] = 0·009; P*[CpG/PGE2 10 μm+ CpG] = 0·007; P*[CpG/PGE2 10 μm+ TGF + CpG] = 0·009 (b) TNF.P*[CpG/TGF + CpG] = 0·009; P*[CpG/PGE2 1 μm+ CpG] = 0·007; p*[CpG/PGE2 10 μm + CpG] = 0·03; P*[Medium/PGE2 10 μm+ TGF + CpG] = 0·03; (c) IL-8.P*[CpG/PGE2 10 μm+ CpG] = 0·03.