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FALS–SOD1 metalloproteins retain in vivo copper buffering. (A) sod1Δcup1Δ (JS004) yeast harboring yeast SOD1-promoted FALS–SOD1 expression plasmids were diluted to a low density in minimal media, the indicated level of copper sulfate was added, cultures were permitted to grow for 3 days, and culture growth was measured spectrophotometrically. All values represent the average and range of three independent cultures in a single experiment.
