Figure 5.

FALS–SOD1 mutants depend on the SOD1 copper chaperone for efficient copper loading in vivo. (A) Cells (10⁶) of sod1Δ (VC107) or sod1Δlys7Δ (VC277) yeast harboring integrated SOD1-promoted FALS–SOD1 constructs (Left) or high copy SOD1-promoted plasmids (Right) were spotted onto plates lacking lysine and permitted to grow for 2 days. (B) sod1Δ and sod1Δlys7Δ yeast harboring the high copy PGK–SOD1 constructs were spotted onto SC plates with lysine (Left), without lysine (Center), or without lysine and with 250 μM BCS (Right).