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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Histotechnol. 2009 Mar 1;32(1):27–31. doi: 10.1179/his.2009.32.1.27

Table 1.

Processing schedule

Solution Time in solution Temp
Fixative
 10% NBF or 70% EtOH 16 h RT
Wash
 PBS 15 min RT
Dehydration procedure
 70% Acetone or 70% EtOH 24 h 4°C
 90% acetone 1 h 4°C
 100% acetone 1 h 4°C
 100% acetone 1 h 4°C
Infiltration procedure
 MMA infiltration solution (vacuum dessicator) 48 h 4°C
 MMA embedding solution (waterbath in radiant oven) 48 h 37°C