Fig. 1.

Superior cervical ganglion (SCG) neurons do not endogenously express S100A1 protein. A, left: representative confocal image of a SCG neuron immunostained with anti-S100A1 and counterstained with a secondary Alexa 488-conjugated antibody demonstrates no endogenous expression of S100A1. Right: corresponding transmitted light image. Scale bars, 10 μm. B: representative Western blot of SCG neurons reveals no endogenous S100A1 expression in cultured SCG neurons (lane 1), with purified S100A1 (lane 2) as a positive control.