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. 2009 Jul 29;297(4):C907–C915. doi: 10.1152/ajpcell.00536.2008

Fig. 7.

Fig. 7.

CO and bile pigments prevent HOCl-mediated cytotoxicity in HUVEC. A: cell viability following treatment of HUVEC with HOCl (300 μM for 24 h) in the presence or absence of biliverdin (BV; 10 μM), bilirubin (BR; 10 μM), CORM2 (10 μM), or iron (10 μM). B: effect of HOCl on mitochondrial membrane potential. Cells were treated with HOCl (300 μM for 4 h) in the presence or absence of bilirubin (10 μM) or CORM2 (10 μM) and mitochondrial membrane potential determined in MitoCapture-loaded cells by confocal microscopy (magnification, ×20). C: quantification of the red-to-green fluorescence ratio in cells treated with HOCl (300 μM) in the presence or absence of BR (10 μM) or CORM2 (10 μM). D: caspase-3 activity following treatment of cells with HOCl (300 μM for 24 h) in the presence or absence of BR (10 μM) or CORM2 (10 μM). Results are means ± SE (n = 4–6). *Statistically significant effect of HOCl.