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Characterization of focal adhesion kinase-knockout (FAK−/−) mouse aortic endothelial cells (MAECs). A: aortic endothelial cells from homozygous conditional FAK floxed/floxed mice (FAKfl/fl or fl/fl cells). Lysates were analyzed by Western blotting for FAK, NF-κB p65 subunit, Pyk2, or tubulin as a loading control. B: cells were fixed and stained for paxillin and β-catenin. Scale bars represent 20 μm (×63) or 50 μm (×40).
