Fig. 6.
CO treatments (250 ppm) significantly increased immature hematopoietic cells in S+S-Antilles bone marrow. S+S-Antilles mice (n = 4) were treated with 250 ppm CO 3 days/wk, 1 h/day for 8 wk. After 8 wk, bone marrow from the CO-treated mice and untreated age-matched controls (n = 5) was collected and stained for CD135 (flk2) and CD90.1 (Thy1.1). A and B: representative SSC vs. CD90.1 FACS plots featuring the gating of CD90.1+ populations in control (A) and 250-ppm CO-treated (B) S+S-Antilles mice. C and D: representative SSC vs. CD135 FACS plots for the CD90.1+ populations in control (A) and 250-ppm CO-treated (B) S+S-Antilles mice. E: bar graph demonstrating the significant increase in the percentage of CD90.1+CD135+ cells in mouse bone marrow in 250-ppm CO-treated S+S-Antilles mice compared with control S+S-Antilles mice. Values are mean %positive cells ± SE. *P < 0.001 compared with controls using two-tailed t-test.