Fig. 2.
SAP97 increases whole cell Kir2.3 currents in HEK293 cells without changing rectification or pH sensitivity. A: Kir2.3 currents were measured as barium (1 mM)-sensitive currents (control current − barium current), and membrane potentials reflect command voltages of the amplifier. Inset: voltage protocol. B: Kir2.3 currents measured in the Kir2.3 stable cell line with or without coexpression with SAP97 (Kir2.3, N = 5; Kir2.3 + SAP97, N = 6). C: rectification of Kir2.3 current in control and following coexpression with SAP97 (Kir2.3, N = 5; Kir2.3 + SAP97, N = 6). D: Kir2.3 current in the presence of SAP97 is inhibited by decreased pHo. Left: current density in control and at pHo = 6.0. Right: average peak inward (filled bars) and outward (open bars) current density in control and at pHo = 6.0 (N = 3).