Fig. 8.

Total amount of SAP98 in SAP97-transfected HEK cells was compared with the endogenous SAP97 levels in this cell line using Western blot analysis, with sheep atrial homogenate serving as a positive control. In all samples, the anti-SAP97 antibody detected a single band of the appropriate size. As expected, SAP97-transfected HEK cells expressed substantially more of the scaffolding protein than the nontransfected cells, with expression levels comparable to those seen in 24 μg of sheep atrial homogenate. For this amount of sheep atrial proteins, β-actin immunoreactivity was not detectable at the exposure used for the HEK cell samples.