Fig. 5.

Epifluoresence imaging of functional retrograde uptake and internalization of acetylated low-density lipoprotein (AcLDL), transferrin (Tfn), and cholera toxin B (CTB) in PAECs exposed to MCTP and c-PTIO for 4 days. Respective fluorescent ligand uptake and internalization assays were carried out in PAEC cultures 4 days after commencement of MCTP or c-PTIO treatment using procedures summarized in Materials and Methods with variations as indicated in the figure. Multiple epifluoresence images (×40 objective, n = 4–6 per variable) were collected in each experiment. A–E: representative illustrations of ×4 zoomed-in sections of representative image frames from separate experiments; full ×40 objective image frames corresponding to these experiments are illustrated in Supplemental Fig. 3. Scale bars = 12.5 μm.