Table 1.
Similar and dissimilar subcellular changes in PAECs exposed to MCTP or NO scavenging with c-PTIO
| Feature | MCTP | c-PTIO |
|---|---|---|
| Cell morphology | enlarged, angular | enlarged, cuboidal |
| Entry into mitosis | inhibited (inhibits that of c-PTIO) | enhanced |
| Nuclear size | increased | increased, reduced postmitosis |
| Cell surface NO (DAF2-DA) | inhibited | inhibited |
| S-nitrosylation* | ||
| Caveolin-1 | inhibited | inhibited |
| Clathrin heavy chain | inhibited | inhibited |
| NSF | inhibited | inhibited |
| eNOS | inhibited | inhibited |
| Golgi (giantin, GM130, p115) | increased, enlarged | increased, dispersed |
| Protein localizations | ||
| caveolin-1 | reduced plasma membrane, some in Golgi (GM130) | reduced plasma membrane, some in Golgi (GM130) |
| eNOS | reduced plasma membrane, increased cell-centric | increased at intercellular junctions, cell-centric |
| PECAM-1 (CD31) | loss from intercellular contacts | loss from intercellular contacts |
| β-actin | increased stress fibers | cell-periphery |
| Live-cell secretion (ssHRP)† | initial inhibition, then enhanced, long-lived | initial inhibition, then enhanced, long-lived |
| Live-cell compartment labeling† | ||
| C5 ceramide (Golgi) | enhanced | inhibited |
| LysoTracker | unchanged | unchanged |
| ER-Tracker | unchanged | unchanged |
| MitoTracker | unchanged | unchanged |
| Live-cell ligand uptake2 | ||
| AcLDL | inhibited | inhibited |
| Transferrin | inhibited | inhibited |
| CTB | inhibited | inhibited |
| Surface-accessible receptor† | ||
| LDLR | reduced | unchanged |
| Transferrin-R | reduced | small reduction |
| PECAM-1 (CD31) | reduced | reduced |
| BMPR-2 | reduced | small reduction |
| Tie-2 | reduced | small reduction |
c-PTIO, (4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; MCTP, monocrotaline pyrrole; DAF-2DA, 4-5 diaminofluorescein diacetate; HRP, horseradish perioxidase; NSF, N-ethylmaleimide-sensitive factor; PECAM-1, platelet endothelial cell adhesion molecule-1; AcLDL, acetyl low density lipoprotein; CTB, cholera toxin subunit; BMPR-2, bone morphogenetic receptor type-2.
*
From Ref. 38.
†
Compared with untreated PAECs on a per unit cell surface basis.