Table 1.
Microvascular and macrovascular pulmonary endothelial cells display contrasting migratory behavior
| DMEM | 10% FBS | VEGF | ET-1 | S-1-P | PAR1-AP | PAR2-AP | |
|---|---|---|---|---|---|---|---|
| RPMVEC | |||||||
| Collagen | 1.0±0.3 | 44.6±9.9 | 0.7±0.4 | 1.1±0.1 | 0.8±0.8 | ||
| Fibronectin | 11.1±2.3 | 52.4±8.6* | 9.4±4.3 | 9.1±6.3 | 9.7±3.5 | ||
| RPAEC | |||||||
| Collagen | 19.5±3.0 | 100.3±1.2* | 38.3±3.3* | 23.4±3.7 | 45.9±1.4* | 66.1±11.4* | 53.3±4.0* |
| Fibronectin | 21.9±2.1 | 105.9±3.4* | 40.8±5.4* | 23.9±3.2 | 54.9±4.6* | 57.9±3.5* | 59.9±7.0* |
Values are mean cells per high-powered field ± SD, n = 3. Rat pulmonary microvascular endothelial cells (RPMVEC) did not migrate in response to classic endothelial cell agonists: VEGF (vascular endothelial growth factor, 100 ng/ml); basic fibroblast growth factor (100 ng/ml); ET-1 (endothelin-1, 10−7 mol/l); S-1-P (sphingosine-1-phosphate, 10−6 mol/l). In contrast, rat pulmonary artery endothelial cells (RPAEC) migrated with classic agonists compared with DMEM. Migration was insensitive to the extracellular matrix coating [agonist concentrations same as above; protease-activated receptor 1 agonist peptide (PAR1-AP) (SFLLRN, 10−4 mol/l); PAR2-AP (SLIGRL, 10−4 mol/l].
P < 0.05 vs. DMEM.