Fig. 4.
Effects of PTHrP on cell cycle proteins. PTHrP transfection decreased levels of cyclin D2 and cyclin A2 (A), while increasing expression of the p27Kip1 cyclin-dependent kinase (CDK) inhibitor (B). The CDKs, cyclin B1, cyclin D1, cyclin D3, and cyclin E2, were unaffected by PTHrP. Representative immunoblots appear on the left, and bar graphs of relative density, normalized by the densities of the actin bands, on the right. Data represent means ± SE from 3 independent clones per experimental group. *P < 0.05, **P < 0.01 vs. vector control. Blots here and in other figures were performed multiple times with reproducible results.