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. Author manuscript; available in PMC: 2009 Oct 30.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Feb 22;49(6):2599–2605. doi: 10.1167/iovs.07-1508

Figure 7.

Figure 7

Immunoblotting analysis of WT and LOXL1 mutant retina/choroid tissues before and 7 days after treatment with laser. (A) Soluble elastin (tropoelastin) and VEGF were found at much higher levels in the mutant tissues after laser treatment. With this assay, tropoelastin was also elevated in the LOXL1 mutant before laser treatment, but VEGF was not detected (data not shown). (B) There is an apparent increase of MT1-MMP in the LOXL1 mutant after laser treatment. Only the detergent-soluble fractions are shown. Top: before laser treatment. Middle: after laser treatment. (A, B, bottom) Acetylated (Ac) tubulin as a loading control. (C) Quantitation of MT1-MMP immunoblots (n = 3) showing a significant increase in MT1-MMP levels in the mutant over WT eyes after laser treatment.