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Example of using a 10 × 10 matrix to screen for malaria infection. Mini-pools (squares) are made up of 10 samples (circles) each. The octagon represents a master-pool made from 10 mini-pools. A negative master-pool would indicate that all 100 samples constituting the pool are negative, precluding further testing. The black squares and circles represent PCR-positive samples. The gray circles represent negative samples that need to be tested individually to rule out infection.
